机构概况
机构简介   现任领导
历任领导   学术委员会
学位委员会   组织机构
两院院士   历史沿革
园区风貌   历年年报
人才招聘   信息公开
研究队伍
院士专家
研究组长
万人计划
千人计划
杰出青年
百人计划
优青
博士后流动站
人才招聘
 您现在的位置:首页 > 研究队伍
研究队伍
姓 名:
 万亚坤
性 别:
 男
职 称:
 研究员
学 历:
 博士
电 话:
 021-50800127
传 真:
 
电子邮件:
 ykwan@simm.ac.cn
个人主页:
通讯地址:
  
简历:

  2004年在中国科学院植物所获得理学博士学位。2004年至2009年分别在美国德克萨斯大学奥斯丁分校、美国贝勒医学院和美国系统生物学研究所从事博士后研究,2009年至2010年在美国系统生物学研究所担任研究员,2010年至2015在东南大学生命科学研究院担任教授。2015年入选中科院药物所“百人计划”,受聘于上海药物研究所,担任课题组长。

  课题组工作重点主要集中在纳米抗体(Nanobody)用于治疗性抗体药物及疾病临床诊断方面的研究。2014年担任“江苏省纳米抗体工程技术研究中心”主任。近年来,共发表了46篇论文,申请纳米抗体相关发明技术专利27项,授权8项。

教育经历:

  2009-2010 美国系统生物学研究所,西雅图,美国,研究员

  2006-2009 美国系统生物学研究所,西雅图,美国,博士后

  2005-2005 美国德克萨斯大学奥斯丁分校,奥斯丁,美国,博士后

  2001-2004 中国科学院植物研究所,理学博士

  1998-2001 西北农林科技大学&第四军医大学,理学硕士

  1994---1998 石河子大学,植物学学士

工作经历: 

  2015-至今 江苏省纳米抗体工程技术研发中心,主任

  2015-至今 中国科学院上海药物研究所,课题组长,博士后导师

  2010-2015 东南大学生命科学研究院担任教授,纳米抗体生物工程课题组长


研究方向:
1. 新型基因工程纳米抗体筛选平台的建设;
2. 肿瘤与自身免疫病治疗性纳米抗体的研发;
3. 基于纳米抗体的疾病检测新技术新方法的产业化。

专家类别:
研究员;百人计划

职务:
课题组长

科研成果:

  近年来,共发表了46篇论文,其中SCI英文期刊论文43篇,影响因子一共为217.3。其中第一作者及通讯作者共33篇,影响因子一共为138.5(影响因子按2015年统计结果)。承担了国家自然科学基金面上项目4项。申请纳米抗体相关发明技术专利27项,授权8项。


承担科研项目情况:

1.国家自然科学基金委面上项目,81673344、全新肿瘤免疫治疗药物CTLA-4阻断型纳米抗体的抗肿瘤作用研究、2017/1-2020/12、在研、主持。

2.国家自然科学基金委面上项目,31471216、组蛋白分子伴侣调控H2A.Z在全基因组分布的分子机制、2015/1-2018/12、在研、主持。

3.国家自然科学基金委面上项目,31271365、组蛋白H3变体Cse4在着丝粒的精确定位对维持基因组稳定性的分子机制研究、2013/1-2016/12、在研、主持。

4.国家自然科学基金委面上项目,31071146、细胞核孔复合体调控亚端粒区域基因沉默的分子机制研究、2011/1-2013/12、结题、主持。

5.教育部新世纪优秀人才支持计划,NCET-13-0127、细胞衰老的表观遗传调控、2014/6-2016/12、在研、主持。

6.江苏省自然科学基金面上项目,BK2011599、亚精胺延长细胞寿命的作用机制、2011/9-2013/9在研、主持。


社会任职:

1.2007-至今 美国细胞生物学学会会员

2.国际期刊“Biosensors & bioelectronics”专家审稿人

3.国内核心期刊“微生物学报”、“微生物学通报”编委、专家审稿人


获奖及荣誉:

1.2004年 中科院地奥奖学金二等奖

2.2003年 中国科学院植物研究所一等奖

3.2001年 西北农林科技大学优秀毕业研究生奖

4.1998年 石河子大学优秀毕业大学生奖


代表论著:

1.Van de Vosse DW*, Wan Yakun*, Lapetina DL, Chen WM, Chiang JH, Aitchison JD, Wozniak RW. 2013, A role for the nucleoporin Nup170p in chromatin structure and gene silencing. Cell, 152(5):969-983.(*Co-first author)

2.Wan Yakun, Chan-Hsien Lin, Nina E. Arens, Jennifer Smith, Ramsey A. Saleem, Jung-Hsien Chiang, John D. Aitchison. 2010, Histone chaperone Chz1p regulates telomeric anti-silencing by modulating H2B ubiquitination at telomeric chromatin. Nucleic Acids Research, 38(5):1431-1440

3.Li H, Yan J, Ou W, Liu H, Liu S, Wan Yakun*. 2015. Construction of a biotinylated cameloid-like antibody for lable-free detection of apolipoprotein B-100. Biosensors & bioelectronics, 64:111-118

4.Ma L, Sun Y, Kang X, Wan Yakun*. 2014. Development of nanobody-based flow injection chemiluminescence immunoassay for sensitive detection of human prealbumin. Biosensors & bioelectronics, 61:165-71

5.Wang H, Li G, Zhang Y, Zhu M, Ma H, Du B, Wei Q, Wan Yakun*. 2015, Nanobody-Based Electrochemical Immunoassay for Ultrasensitive Determination of Apolipoprotein-A1 Using Silver Nanoparticles Loaded Nanohydroxyapatite as Label. Analytical chemistry, 7(22):11209-14.

6.Liu H, Wang P, Liu L, Min Z, Luo K, Wan Yakun*.2015, Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae. Scientific Reports, 5:15583.

7.Henan Li, Yawen Mu, Junrong Yan, Yakun Wan*, Songqin Liu. 2015. Label-free photoelectrochemical immunosensor for neutrophil gelatinase-associated lipocalin based on the use of nanobodies. Analytical Chemistry, 87(3):2007-15.

8.Wan Yakun, Saleem RA, Ratushny AV, Roda O, Smith JJ, Lin CH, Chiang JH, Aitchison JD. 2009, Role of the histone variant H2A.Z/Htz1p in TBP recruitment, chromatin dynamics, and regulated expression of oleate-responsive genes. Molecular and Cellular Biology, 29(9):2346-2358

9.Mi L, Wang P, Yan J, Qian J, Lu J, Yu J, Wang Y, Liu H, Zhu M, Wan Yakun*, Liu S. 2016, A novel photoelectrochemical immunosensor by integration of nanobody and TiO2 nanotubes for sensitive detection of serum cystatin C. Analytica chimica acta, 902:107-14.

10.Yan J, Wang P, Zhu M, Li G, Romao E, Xiong S, Wan Yakun*. 2015, Characterization and applications of Nanobodies against human procalcitonin selected from a novel na?ve Nanobody phage display library. Journal of Nanobiotechnology, 6;13:33.

11.Wan Yakun*, Arens CE, Wang S, Zuo X, Zhuo Y, Xing J, Liu H. 2013, Role of the repressor Oaf3p in the recruitment of transcription factors and chromatin dynamics during the oleate response. Biochemical Journal, 449(2):507-517.

12.Li H, Sun Y, Elseviers J, Muyldermans S, Liu S, Wan Yakun*. 2014. A nanobody-based electrochemiluminescent immunosensor for sensitive detection of human procalcitonin, Analyst, 139(15):3718-21.

13.Liu H, Luo K, Zhou Z, Mu Y, Wan Yakun*. 2014. Histone chaperone Chz1 facilitates the disfavouring property of Spt16 to H2A.Z-containing genes in Saccharomyces cerevisiae. Biochemical Journal, 460(3):387-97 (*通讯作者)

14.Yan J, Li G, Hu Y, Ou W, Wan Yakun*. 2014. Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications. Journal of Translational Medicine, 12(1):343.

15.Min Zhu, Xue Gong, Yonghong Hu, Weijun Ou, Yakun Wan*. 2014. Streptavidin-biotin-based directional double nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1. Journal of Translational Medicine, 20;12:352.

16.Min Zhu, Yonghong Hu, Guirong Li, Weijun Ou, Panyong Mao, Shaojie Xin, Wan Yakun*. 2014. Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2. Nanoscale Research Letters, 9(1):528

17.Liu H, Zhu Min, Mu Y, Liu L, Li G, Wan Yakun. 2014. Distinct roles for histone chaperones in the deposition of HTZ1 in chromatin. Bioscience Reports, 34(5):139

18.Wang P, Li G, Yan J, Wan Yakun. 2014. Fabrication of Biotinylated Camel Nanobody Based Immunoassay for Specific and Sensitive Detection of Cry1F Toxin. Toxicon, 92C:186-192

19.Li M, Zhu M, Zhang C, Liu X, Wan Yakun. 2014. Uniform Orientation of Biotinylated Nanobody as an Affinity Binder for Detection of Bacillus thuringiensis (Bt) Cry1Ac Toxin. Toxins (Basel), 6(12):3208-22

20.Gong X, Zhu M, Li G, Lu X, Wan Yakun*.2015, Specific determination of influenza H7N2 virus based on biotinylated single-domain antibody from a phage-displayed library. Analytical biochemistry, S0003-2697(15)00462-5.

21.Liu H, Li G, Liu L, Wan Yakun*. 2015, Functional role of histone variant Htz1 in the stress response to oleate in Saccharomyces cerevisiae. Bioscience reports, 35(4). pii: e00224.

22.Mu Y, Xie H, Wan Yakun*. 2015, Sensitive and Specific Neutrophil Gelatinase-associated Lipocalin Detection by Solid-phase Proximity Ligation Assay. Analytical sciences, 31(6):475-9.

23.Zijun Sun, Yanyan Sun, Yaxian Zhou and Yakun Wan*. 2012. Yeast geneomics technique for high-throughput drug target discovery. Drug Development Research, 73:398-405.

24.Wan Yakun, Weiming Chen, Xing Jie, Tan Jinyin. 2011. Transcriptome profiling to reveal novel roles of Trichostatin A in antagonizing histone chaperone Chz1 mediated telomere anti-silencing. FEBS letters, 585(15):2519-2525

25.Wan Yakun, Tian Shiping. 2002. Antagonistical mode of yeast Pichia membranefaciens to Rhizopus stolonifer in wounds of peach fruit by scanning electron microscope. Journal of Integrative Plant Biology, 44:1384-1386

26.Wan Yakun, Zuo X, Zhuo Y, Zhu M, Danziger SA, Zhou Z. 2013, The functional role of SUMO E3 ligase Mms21p in the maintenance of subtelomeric silencing in budding yeast. Biochemical and biophysical research communications, 438(4):746-52.

27.Wan Yakun*, David Van de Vosse*, Richard Wozniak, John Aitchison, 2011. Effects of nuclear architecture on genome function, Wiley Interdisciplinary Reviews: Systems Biology and Medicine, 3(2):147-166 (*Co first author)

28.Wan Yakun, Tian Shiping. 2005. Integrated control of postharvest diseases of pear fruits using antagonistic yeasts in combination with ammonium molybdate. Journal of the Science of Food and Agriculture, 85 (15): 2605-2610

29.Wan Yakun, Tian Shiping. 2005. G418-resistance as a dominant selectable marker for heterogenous gene expression in antagonist Pichia membranefaciens. Agricultural Sciences in China, 4(1): 101-105

30.Wan Yakun, Tianshiping. 2004. Optimization of media for production of an effective yeast biocontrol agent Pichia membranefaciens. Agricultural Sciences in China, 3(4):286-291

31.Wan Yakun, Tian Shiping, Qin Guozheng. 2003. Enhancement of biocontrol activity of yeasts by adding sodium bicaronate or ammonium molybdate to conrtol postharvest disease of jujube. Letters in applied microbiology, 37:249-253

32.Chen Huiying, Zhang Jiazhen, Wan Yakun*. 2012. Molecular mechanisms of histone variant Htz1 action in the transcriptional regulation in Saccaromyces cerevisiae. Chinese Journal of Biochemistry and Molecular Biology, 28(2):152-158(*通讯作者)

33.Zhang Jiazhen, Tan Jinyin, Zuo Xiao, Li Bin, Lv Zhaoxia, Zhao Ruipu, Wan Yakun*. 2011. Progress on Molecular Mechanisms of Chronological ageing in model organism Saccaromyces cerevisiae. Progress in Biochemistry and Biophysics, 38(2):119-126

34.Theo Knijnenburg*, Roda Orial*, Wan Yakun, Garry P. Nolan, John D. Aitchison, Ilya Shmulevich1. 2011. A regression model approach to enable cell morphology correction in high throughout flow cytometry. Molecular Systems Biology, 7:531-543

35.Li HN, Mu YW, Lu JS, Wei W, Wan Yakun, Liu SQ. 2014. Target-Cell-Specific Fluorescence Silica Nanoprobes for Imaging and Theranostics of Cancer Cells. Analytical chemistry, 86(7):3602-9

36.Danziger SA, Ratushny AV, Smith JJ, Saleem RA, Wan Yakun, Arens CE, Armstrong AM, Sitko K, Chen WM, Chiang JH, Reiss DJ, Baliga NS, Aitchison JD. 2014. Molecular mechanisms of system responses to novel stimuli are predictable from public data. Nucleic Acids Research, 42(3):1442-60

37.Du Fuying, Liu Ting, Liu Tian, Wang Yongwei, Wan Yakun, Jie Xing. 2011. Metabolite identification of triptolide by data-dependent accurate mass spectrometric analysis in combination with online H/D exchange and multiple data mining techniques. Rapid Communication Mass Spectrometry,25(20): 3167-3177

38.DuBois KN, Alsford S, Buisson J, Swiderski M, Bart JM, Ratushny AT, Wan Yakun, Bastin P, Barry JD, Navarro M, Horn D, Aitchison JD, Rout MP, Field MC, 2012. Epigenetic regulation at the nuclear periphery mediated by a non-metazoan lamin analogue. PloS Biology, 10(3):e1001287

39.Liu Tian, Du Fuying, WanYakun, Zhua Fanping, Xing Jie. 2011. Rapid identification of phase I and II metabolites of artemisinin antimalarials using LTQ-Orbitrap hybrid mass spectrometer in combination with online hydrogen deuterium exchange technique. Journal of Mass Spectrometry, 10;46(8):7257-33

40.Smith JJ, Miller LR, Kreisberg R, Vazquez L,Wan Yakun, Aitchison JD. 2011. Environmental responsive transcription factors bind subtelomeric elements and regulate gene silencing. Molecular Systems Biology, 4:455-466

41.Saleem RA, Ratushny AV, Rogers RS, Dilworth DJ, Shannon PT, Shteynberg D, Wan Yakun, Morirz RL, Nesvizhskii AL, Rachubinski RA, Aitchison JD. 2010, Integrated Phosphoproteomic analysis reveals conservation of scale free properties in a nutrient responsive signaling network. Molecular and Cellular Proteomics, 9(9):2076-2088

42.Saleem RA, R Long O’Donnell, DJ Dilworth, AM Armstrong, A Jamakhandi, Wan Yakun, TA, Knijnenburg, A Niemist?, J Boyle, RA Rachubinski, I Shmulevich, JD Aitchison, 2010, Genome-Wide Analysis of Effectors of Peroxisome Biogenesis, PloS One, 5: e11953

43.Ratushny AV, Ramsey SA, Roda O, Wan Yakun, Smith JJ, Aitchison JD. 2008, Control of transcriptional variability by overlapping feed-forward regulatory motifs. Biophysical Journal, 95(8): 3715-3723

44.Joglekar AP, Bouck D, Finley K, Liu Xingkun, Wan Yakun, Berman J, He Xiangwei, Salmon ED, Bloom KS. 2008, Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres. The Journal of Cell Biology, 181(4):587-594

45.Tian Shiping, Wan Yakun, Qin Guozheng, Xu yong.2006, Induction of defense responses against Alternaria rot by different elicitors in harvested pear fruits. Applied Microbiology and Biotechnology, 70(6):729-734

46.Qin Guozheng, Tian Shiping, Xu Yong, Wan Yakun. 2003. Enhancement of biocontrol efficacy of antagonistic yeasts by salicylic acid in sweet cherry fruit. Physiological and Molecular Plant Pathology, 62:147-154